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KMID : 0613820180280080908
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Journal of Life Science
2018 Volume.28 No. 8 p.908 ~ p.916
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Ethanolic Extract of Oryza sativa Displays Antioxidative Activity and Promotes Melanin Synthesis
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Jeon So-Jeong
Kim Moon-Moo
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Abstract
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Hair loses melanin with aging, which leads to hair graying. The change in hair color is caused by a reduction in tyrosinase activity and an accumulation of hydrogen peroxide (H2O2) in hair follicles. The purpose of this study was to investigate the effect of ethanolic extract of Oryza sativa (OREE) on melanin production and antioxidative activity in B16F1 cells. In this study, OREE showed low DPPH radical scavenging activity and reducing power. However, it displayed a strong antioxidative effect against intracellular H2O2 in live cells. OREE did not inhibit DOPA oxidation activity in vitro, but it increased tyrosinase activity at a concentration of 64 μg/ml. OREE at a concentration higher than 32 μg/ml showed cell toxicity in B16F1 cells. However, OREE at a concentration higher than 8 μg/ml not only increased melanin synthesis in a dose-dependent manner in B16F1 cells but also increased melanin synthesis in cells treated with H2O2 inhibiting melanin synthesis. To confirm the effect of OREE on melanin production, Western blot analysis was performed. The results revealed that OREE increased the expression levels of tyrosine hydroxylase and tyrosinase-related protein-2 (TRP-2) involved in melanin production in the H2O2-treated cells in which melanin production was inhibited. The findings suggest that OREE could improve melanin synthesis and be available for development of hair cosmetics aimed at improving melanin production.
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KEYWORD
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H2O2, melanin, Oryza sativa, tyrosine hydroxylase, TRP-2
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